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DNA and siRNA transfection reagent

ScreenFect™A

A New liposome developed by click chemistry!!

ScreenFect™A is a transfection reagent consisting of a new cationic liposome screened*1 by click chemistry. It can be used with various eukaryote-derived cells and can be added directly to mediums containing antibiotics or serum. DNA and siRNA can be transfected into general experimental cell strains (HeLa, HepG2, MDCK, Cos-7, etc.), stem cells (mouse ES cells, etc.), blood cells (macrophages, THP-1, RAW264, 7, etc.), microglia, primary (initial subculture) cells, and Insect cell. Medium replacement after transfection is not required due to low cytotoxicity. The constituent reagents do not contain any poisonous or deleterious substance.

*1 Biomaterials. 2012 Nov; 33(32):8160-6. 2012

  • High transfection efficiency and low cytotoxicity
  • Can be used for both DNA and siRNA
  • No need to exchange medium and can be used in the presence of serum

ScreenFect™ Sample
Ordering form.

Please fill out the answers as much as possible. We will give the sample pack of ScreenFect™.

ScreenFect™
Sample Ordering form

Questionnaire after the
ScreenFect™ has been used.

Please fill out the answers as much as possible. We will give a small gift to the people who complete the questionnaire.

Questionnaire after
the ScreenFect™ has been used.

In comparison with competitor

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GFP-expressing plasmid DNA was transfected into HEK293 cells using ScreenFect™A. The result demonstrated transfection efficiency is equal or superior to competitors. (96-well plate, GFP-expressing plasmid DNA 75 ng/well)

Transfection efficiency of liposome library

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GFP-expressing plasmid DNA was transfected into HEK293T cells using a new cationic liposome library synthesized by click chemistry. As a results, a new liposome (ScreenFect™A) which can transfect plasmid DNA more efficient than company A was confirmed.

Outline of ScreenFect™A protocol

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The ideal mixing ratio of DNA/siRNA and transfection reagent varies depending on the type of cells.
We will recommend you to examine several ratios and choose the best one.
           
DNA transfection
DNA transfection (/well)
Plate size Surface area Medium volume Total volume SF-DNA complex DNA / Dilution Buffer ScreenFect™A / Dilution Buffer
96 wells 0.3cm2 100μl 10μl ~100ng / 5μl ~0.6μl / 5μl
24 wells 2cm2 500μl 50μl ~500ng / 25μl ~3μl / 25μl
12 wells 4cm2 1000μl 100μl ~1000ng / 50μl ~6μl / 50μl
6 wells 10cm2 2000μl 250μl ~2500ng / 125μl ~15μl / 125μl
siRNA transfection
siRNA transfection (/well)
Plate size Surface area Medium volume Total volume SF-siRNA complex siRNA / Dilution Buffer ScreenFect™A / Dilution Buffer
96 wells 0.3cm2 100μl 10μl ~3pmol / 5μl ~0.45μl / 5μl
24 wells 2cm2 500μl 50μl ~20pmol / 25μl ~2μl / 25μl
12 wells 4cm2 1000μl 100μl ~40pmol / 50μl ~4μl / 50μl
6 wells 10cm2 2000μl 250μl ~90pmol / 125μl ~10μl / 125μl

Please refer to the manual provided with the product for further information of usage.
If you need the protocol of optimization according to the type of cells, feel free to ask us.

Transfection data

Low cytotoxicity

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GFP-expressing plasmid DNA was transfected into HEK293 cells using ScreenFect™A. The results demonstrated gene transfection efficiency is equal or superior to competitors. Cytotoxicity was also comparable to competitors. (96-well plate, GFP-expressing plasmid DNA 75 ng/well)

Gene transfection into mouse ES cells

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GFP-expressing plasmid DNA was transfected into mouse ES cells using ScreenFect™A and GFP-positive cells were detected. As a result, about 60% or more of mouse ES cells were GFP-positive cells.

About DNA transfection
Also applicable to gene transfection into stem cells!


siRNA transfection

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LRP6 siRNA was transfected into HEK293 cells using ScreenFect™A. It showed that a higher knockdown efficiency than the Company A and the Company B. (96-well plate, final concentration of 1 pmole LRP6 siRNA at 2 nM/well)

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GAPDH siRNA was transfected into each cell line using ScreenFect™A. The results demonstrated that a higher knockdown efficiency than competitor. (96-well plate, final concentration of GAPDH siRNA at 3 nM/well)

siRNA transfection
applicable to siRNA transfection!!

List of cells transfected by ScreenFect™A or A plus

For further information, refer to the ScreenFect™ Databese.

No. Name of Cell
1 143BTK
2 786-O
3 A2058
4 A375
5 A549
6 B16
7 B16F10
8 Ba/F3-CH1
9 BEAS-2B
10 BEL-7402
11 BT549
12 C2C12
13 Cell line from killifish
14 CHO-K1
15 COS-7
16 DB lymphoma
17 DC 2.4
18 Du145
19 EL4
20 Endothelium cell
21 EPC(carp)
22 GP2-293
23 H9C2
24 HCT116
25 HEK293
26 HEK293 TN
27 HEK293A
28 HEK293F
29 HEK293FT
30 HEK293T
31 HeLa
32 HeLa S3
33 HEp-2
34 HepG2
35 hiPSC
36 HK2
37 HKC
38 HL7704
39 HuH-7
40 HUVEC
41 Ins-1
42 L428
No. Name of Cell
43 LLC-MK2
44 LO2
45 LoVo
46 MC3T3
47 MC3T3-E1
48 MCF-10
49 MCF-10A
50 MCF-7
51 MDCK
52 MEF
53 mES
54 mHSC
55 Microglia
56 MLEC
57 MS-1
58 Myeloid dendritic cell (MDC)
59 NB1RGB
60 NCI-H1703
61 NE3
62 NIH 3T3
63 NK92
64 OLHNI-2
65 Mouse overy cell
66 PC12
67 Plat-E
68 PLC8024
69 Primary Fibroblast
70 RAW264.7
71 SH-SY5Y
72 SK-Hep1
73 SKOV3
74 T98G
75 TE-13
76 THP-1
77 U-251 MG
78 U2OS
79 U937
80 Vero
81 Drosophira ovary somatic cell
82 HT1080
83 RH7777
84 HaCaT

Wako Pure Chemical Industries, Ltd. is updating constantly the ScreenFect™ Databese. If you are not sure whether it is applicable to cells other than listed above, feel free to ask us.

ScreenFect™A product information

Sales campaign now! We'll offer special price during the campaign.
[Term of campaign : November 1, 2016 to February 28, 2017 ] For further details, inquires to our distributors.

Code No. Product name ScreenFect™A Transfection Reagent Dilution Bufferfor ScreenFect™A Price (JPY)
293-73201 ScreenFect™Aicon 0.2ml 10ml 8,000
299-73203 ScreenFect™Aicon 1ml 50ml 30,000
297-73204 ScreenFect™Aicon 1ml×5 50ml×5 120,000
  • icon ∙∙∙ Store at 2 to 10°C
  • icon ∙∙∙ Store at −20°C
  • icon ∙∙∙ Store at −80°C
  • Store at room temperature if not specified.
  • icon ∙∙∙ Specified poisonous substance
  • iconicon ∙∙∙ Poisonous substance
  • iconiconicon ∙∙∙ Deleterious substance
  • icon ∙∙∙ Class 1 Specified Chemical Substance by the Evaluation of Chemical Substances and Regulation of Their Manufacture, etc. (CSCL)
  • icon ∙∙∙ Class 2 Specified Chemical Substance by the CSCL
  • icon ∙∙∙ Poisonous substance
  • icon ∙∙∙ Deleterious substance
  • icon ∙∙∙ Class 1 designated substance by the Act on the Prohibition of Chemical Weapons and the Regulation of Specific Chemicals,
  • icon ∙∙∙ Class 2 designated substance by the same act
  • icon ∙∙∙ Psychotropic
  • icon ∙∙∙ Raw material of specified narcotics and psychotropics
  • icon ∙∙∙ Cartagena Protocol

* The details given show the information available as of October 2016. Please refer to siyaku.com (http://www.siyaku.com/) for any laws and up-to-date information other than those mentioned above.

The reagent given is intended only for the purposes of testing and research and cannot be used as a “drug”, “food” or “household product”.
Suggested delivery prices show the “price of the product alone” not including consumption tax.
Suggested delivery prices are the prices at the time of preparation of this article. Please check up-to-date prices using the code No. in “Product search”.

ScreenFect™ References
  1. Diefenbacher, Markus E., et al. "The LIM Domain Protein nTRIP6 Recruits the Mediator Complex to AP-1-Regulated Promoters." PLoS ONE 9.5 (2014): e97549.
  2. Freise, Christian, and Uwe Querfeld. "Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34." Pharmacological Research (2014).
  3. Hagiwara, Akane, et al. "Luteinizing Hormone-Induced Expression of Ptger4b, a Prostaglandin E2 Receptor Indispensable for Ovulation of the Medaka Oryzias latipes, Is Regulated by a Genomic Mechanism Involving Nuclear Progestin Receptor."
  4. Peng, Yanyan, Ruidan Xu, and Xiaofeng Zheng. "HSCARG Negatively Regulates the Cellular Antiviral RIG-I Like Receptor Signaling Pathway by Inhibiting TRAF3 Ubiquitination via Recruiting OTUB1." PLoS pathogens 10.4 (2014): e1004041. (3)
  5. Wakimoto, Hiroaki, et al. "Targetable signaling pathway mutations are associated with malignant phenotype in IDH-mutant gliomas." Clinical Cancer Research (2014). (2)
  6. Fischer, Simon, et al. "Breaking limitations of complex culture media: Functional non-viral miRNA delivery into pharmaceutical production cell lines." Journal of biotechnology 168.4 (2013): 589-600.
  7. Bai, Dongmei, et al. "Regulation of the HDM2-p53 pathway by ribosomal protein L6 in response to ribosomal stress." Nucleic acids research 42.3 (2014): 1799-1811.
  8. Liu, Xing, et al. "Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi‐specific monoclonal antibody MsMab‐1." Cancer medicine 2.6 (2013): 803-814.

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