mRNA & Long RNA transfection reagents
New Liposome developed by click chemistry
ScreenFect™mRNA is a transfection reagent consisting of a new cationic liposome by click chemistry screening system*1. ScreenFect™mRNA is suitable for transfection into eukaryotic cells (HeLa, HEK293, MDF cells, etc.) without the replacement of culture medium after transfection because it is not included hazard compounds. ScreenFect™mRNA is used for mRNA transfection procedures.
*1: Biomaterials, Nov.; 33(32): 8160-76, 2012.
- Direct transfection of mRNA
- Long RNA transfection
- Establish iPS cell line without virus vector
Please fill out the answers as much as possible. We will give the sample pack of ScreenFect™.
Sample Ordering form
Questionnaire after the
ScreenFect™ has been used.
Please fill out the answers as much as possible. We will give a small gift to the people who complete the questionnaire.Questionnaire after
the ScreenFect™ has been used.
1.GFP mRNA transfection into HEK293 cells
Transfection conditions of ScreenFect™mRNA
- ・Cell line: HEK293
- ・Protocol: 1-Step method (See below), no-medium replacement
- ・Well format: 96 well plate
- ・Amount of transfection reagent: 0.3 μL/well
- ・Amount of RNA: GFP mRNA [TriLink #L-6101] 75 ng/well
- ・Detection time: 24 hours after the transfection
- ・Detector: Cytation3 microscope [BioTek]
2. EGFP mRNA transfection into HeLa cell (with SFA P-reagent)
Transfection conditions of ScreenFect™mRNA (SF mRNA), SF mRNA + SFA P-reagent
- [Cell number] 0.7x105 cells/well
- [Amount of mRNA] 0.1μg/assay
- [Ratio] mRNA（μg）：ScreenFect™mRNA reagent（μl）= 1：4
- [Amount of SFA P-reagent] 0.2μl/well
mRNA（μg）：SFA P-reagent（μl） = 1：2
- [Well format] 24 well plates
- [Detection time] 48 hours after the transfection
ScreenFect™mRNA transfection conditions
|mRNA transfection (/well) Standard Protocol|
|Plate size||Surface area||Medium volume||Total volume mRNA-lipid complex||Rate of mRNA : Transfection Reagent||mRNA / Dilution Buffer||Transfection Reagent / Dilution Buffer|
|96 wells||0.3cm2||100μl||10μl||1:3||50ng / 5μl||0.15μl / 5μl|
|1:4||0.2μl / 5μl|
|24 wells||2cm2||500μl||50μl||1:3||250ng / 25μl||0.75μl / 25μl|
|1:4||1.0μl / 25μl|
|12 wells||4cm2||1000μl||100μl||1:3||500ng / 50μl||1.5μl / 50μl|
|1:4||2.0μl / 50μl|
|6 wells||10cm2||2000μl||250μl||1:3||1250ng / 125μl||3.75μl / 125μl|
|1:4||5.0μl / 125μl|
Outline of ScreenFect™mRNA protocol
The ideal mixing ratio of mRNA and transfection reagent varies depending on the type of cells.
We will recommend you to examine several ratios and choose the best one.
ScreenFect™mRNA product information
Now on sales campaign! We will offer special price during the campaign.
[Term of campaign : November 11, 2016 to February 28, 2017 ] For further information, inquires to our distributors.
|Code No.||Product name||ScreenFect™mRNA Transfection Reagent||Dilution Buffer for ScreenFect™mRNA||Price (JPY)|
Complete RNA series
The following products are homogeneous mRNAs. Cap structure at the 5' end and a Poly(A) tail at the 3' end are added to the RNA full-length sequence, respectively.
They can be used as templates to obtain control data of absolute quantitation or validation in real-time PCR assay .
|Code No.||Product Name||Pkg. Size||Price (JPY)|
|314-06631||Albumin mRNA, Human||100ng||32,000|
|317-06861||Beta Actin mRNA, Human||100ng||32,000|
|313-06841||GAPDH mRNA, Human||100ng||32,000|
|314-06871||Beta Actin mRNA, Rat||100ng||32,000|
|310-07331||Cebpd mRNA, Rat||100ng||32,000|
|313-07321||Ctss mRNA, Rat||100ng||32,000|
|310-06851||GAPDH mRNA, Rat||100ng||32,000|
|318-06891||Hsd17b2 mRNA, Rat||100ng||32,000|
|311-06881||Hpx mRNA, Rat||100ng||32,000|
|316-07311||Lgals9 mRNA, Rat||100ng||32,000|
|311-06901||Mt1a mRNA, Rat||100ng||32,000|
|318-06911||Orm1 mRNA, Rat||100ng||32,000|
*For the latest price information, access Wako Online Catalog with a click of each Wako Catalog No.
|Product name||Maker Code||Pkg. Size||Price (JPY)|
- ∙∙∙ Store at 2 to 10°C
- ∙∙∙ Store at −20°C
- ∙∙∙ Store at −80°C
- Store at room temperature if not specified.
- ∙∙∙ Specified poisonous substance
- ∙∙∙ Poisonous substance
- ∙∙∙ Deleterious substance
- ∙∙∙ Class 1 Specified Chemical Substance by the Evaluation of Chemical Substances and Regulation of Their Manufacture, etc. (CSCL)
- ∙∙∙ Class 2 Specified Chemical Substance by the CSCL
- ∙∙∙ Poisonous substance
- ∙∙∙ Deleterious substance
- ∙∙∙ Class 1 designated substance by the Act on the Prohibition of Chemical Weapons and the Regulation of Specific Chemicals,
- ∙∙∙ Class 2 designated substance by the same act
- ∙∙∙ Psychotropic
- ∙∙∙ Raw material of specified narcotics and psychotropics
- ∙∙∙ Cartagena Protocol
* The details given show the information available as of October 2016. Please refer to siyaku.com (http://www.siyaku.com/) for any laws and up-to-date information other than those mentioned above.
The reagent given is intended only for the purposes of testing and research and cannot be used as a “drug”, “food” or “household product”.
Suggested delivery prices show the “price of the product alone” not including consumption tax.
Suggested delivery prices are the prices at the time of preparation of this article. Please check up-to-date prices using the code No. in “Product search”.
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- Peng, Yanyan, Ruidan Xu, and Xiaofeng Zheng. "HSCARG Negatively Regulates the Cellular Antiviral RIG-I Like Receptor Signaling Pathway by Inhibiting TRAF3 Ubiquitination via Recruiting OTUB1." PLoS pathogens 10.4 (2014): e1004041. (3)
- Wakimoto, Hiroaki, et al. "Targetable signaling pathway mutations are associated with malignant phenotype in IDH-mutant gliomas." Clinical Cancer Research (2014). (2)
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- Bai, Dongmei, et al. "Regulation of the HDM2-p53 pathway by ribosomal protein L6 in response to ribosomal stress." Nucleic acids research 42.3 (2014): 1799-1811.
- Liu, Xing, et al. "Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi‐specific monoclonal antibody MsMab‐1." Cancer medicine 2.6 (2013): 803-814.
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