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Transfection enhancer reagent

SFA P-reagent

Improve gene transfection efficiency, increase expression ratio and decrease cytotoxity

SFA P-reagent enhances transfection efficiency and expression level in each transfected cells by using ScreenFect™ together when plasmid DNA and mRNA are transfected. Additionally, cytotoxicity also decreases remarkably by adding SFA P-reagent.



SFA P-reagent will resolve the following problems.
  ・Transfection efficiency is low.
  ・Expression level of transfection gene must be increased!
  ・High cytotoxity has been a serious problem.

It's quite easy to use!!
 SFA P-reagent:DNA/mRNA = 2μl:1μg
Mix the reagent with DNA solvent under the ration indicated above.

  • Enhances transfection efficiency
  • Increases the expression level of transfected gene
  • Decreases cytotoxicity
  • Saves the amount of plasmid DNA (Ex. : Half (50%) of current condition)

ScreenFect™ Sample
Ordering form.

Please fill out the answers as much as possible. We will give the sample pack of ScreenFect™.

ScreenFect™
Sample Ordering form

Questionnaire after the
ScreenFect™ has been used.

Please fill out the answers as much as possible. We will give a small gift to the people who complete the questionnaire.

Questionnaire after
the ScreenFect™ has been used.

Application data

1. Experiment of transfecting YFP fusion gene to LNCaP cell (adhesive type)

The experiment of transfection of YFP fusion gene to LNCaP cells (adhesive type) was done, and the expression level of transfected gene was compared by a fluorescence microscope.
Using both ScreenFect™A plus and SFA P-reagent as enhancer reagent, it was demonstrated that the expression efficiency is equal or superior to other competitor’s products.
*Described data in BioWindow No. 144 (published on June, 2016)

In comparison with competitor’s product in the LNCaP cell (human prostatic cancer)

images

[Cell number] 3×105cells/well
[Amount of plasmid DNA] 1µg/assay
[Ratio] pDNA (µg):ScreenFect™A plus reagent(µL)=1:3
[well format] 24 well plates

images

[Cell number] 1.5×105cells/well
[Amount of plasmid DNA] 1µg/assay
[Ratio] pDNA (µg):ScreenFect™A plus reagent(µL)=1:3
[well format] 24 well plates


2. Experiment of transfecting plasmid DNA to HeLa cell

The experiment of transfection of GFP fusion gene to HeLa cells (adhesive type) was done, and the expression level of transfected gene was compared by a fluorescence microscope.
Using both ScreenFect™ A plus and SFA P-reagent, it was demonstrated that the expression efficiency is equal or superior to competitor’s products.

  [Cell number] 1.5×105 cells/well
  [SFA P-reagent] 0.5µL/well
  [Ratio] Plasmid DNA (µg): ScreenFect™A plus reagent(µl) = 1 : 4
  [well format] 24 well plates
  [Detection time] 24 hours after transfection


3. Experiment of transfecting EGFP_mRNA to HeLa cell (adhesive type)

The experiment of transfection of EGFP_mRNA to HeLa cells (adhesive type) was done, and the expression level of transfected gene was compared by a fluorescence microscope.
Using both ScreenFect™A plus and SFA P-reagent as enhancer reagent, it was demonstrated that the expression efficiency is equal or superior to other competitor’s products.
*Described data in BioWindow No. 144 (published on June, 2016)

In comparison with competitor’s product in the LNCaP cell (human prostatic cancer)

  [Cell number] 0.7×105 cells/well
  [Amount of mRNA] 0.1µg/assay
  [Ratio] mRNA(µg) : ScreenFect™A plus reagent(µl) = 1 : 4
  [well format] 24 well plates
  [Detection time] 48 hours after transfection
  [Time of exposure] 2 seconds


4. Data of cytotoxity reduction

The experiment of transfection of luciferase reporter vector to CHO-K1 was done by reverse transfection method (1-step) and compared expression level and cell viability.
The cell viability of cells was increased significantly by adding SFA P-reagent, and expression level was almost as good as that of other company.
*Described data in BioWindow No. 145 (published on September, 2016)

  [Cell number] 2×104 cells/well
  [Amount of plasmid DNA] 100ng/assay
  [Ratio]
     pDNA (µg) : ScreenFect™A plus reagent(µl) = 1 : 3
     pDNA (µg) : SFA P-reagent(µl) = 1 : 2
  [well format] 96 well plates


SFA P-reagent product information

Now on sales campaign! We will offer special price during the campaign.
[Term of campaign : November 11, 2016 to February 28, 2017 ] For further information, inquires to our distributors.

Code No. Product name Quantity Price (JPY)
191-18331 SFA P-reagent  icon 100µL 9,000
197-18333 SFA P-reagent icon 500µL 20,000
  • icon ∙∙∙ Store at 2 to 10°C
  • icon ∙∙∙ Store at −20°C
  • icon ∙∙∙ Store at −80°C
  • Store at room temperature if not specified.
  • icon ∙∙∙ Specified poisonous substance
  • iconicon ∙∙∙ Poisonous substance
  • iconiconicon ∙∙∙ Deleterious substance
  • icon ∙∙∙ Class 1 Specified Chemical Substance by the Evaluation of Chemical Substances and Regulation of Their Manufacture, etc. (CSCL)
  • icon ∙∙∙ Class 2 Specified Chemical Substance by the CSCL
  • icon ∙∙∙ Poisonous substance
  • icon ∙∙∙ Deleterious substance
  • icon ∙∙∙ Class 1 designated substance by the Act on the Prohibition of Chemical Weapons and the Regulation of Specific Chemicals,
  • icon ∙∙∙ Class 2 designated substance by the same act
  • icon ∙∙∙ Psychotropic
  • icon ∙∙∙ Raw material of specified narcotics and psychotropics
  • icon ∙∙∙ Cartagena Protocol

* The information on this page is as of September 16. For legal and the latest information other than the above, please refer to siyaku.com (http://www.siyaku.com/)

The reagent given is intended only for the purposes of testing and research and cannot be used as a “drug”, “food” or “household product”.
Suggested delivery prices show the “price of the product alone” not including consumption tax.
Suggested delivery prices are the prices at the time of preparation of this article. Please check up-to-date prices using the code No. in “Product search”.

ScreenFect™ References
  1. Diefenbacher, Markus E., et al. "The LIM Domain Protein nTRIP6 Recruits the Mediator Complex to AP-1-Regulated Promoters." PLoS ONE 9.5 (2014): e97549.
  2. Freise, Christian, and Uwe Querfeld. "Inhibition of vascular calcification by block of intermediate conductance calcium-activated potassium channels with TRAM-34." Pharmacological Research (2014).
  3. Hagiwara, Akane, et al. "Luteinizing Hormone-Induced Expression of Ptger4b, a Prostaglandin E2 Receptor Indispensable for Ovulation of the Medaka Oryzias latipes, Is Regulated by a Genomic Mechanism Involving Nuclear Progestin Receptor."
  4. Peng, Yanyan, Ruidan Xu, and Xiaofeng Zheng. "HSCARG Negatively Regulates the Cellular Antiviral RIG-I Like Receptor Signaling Pathway by Inhibiting TRAF3 Ubiquitination via Recruiting OTUB1." PLoS pathogens 10.4 (2014): e1004041. (3)
  5. Wakimoto, Hiroaki, et al. "Targetable signaling pathway mutations are associated with malignant phenotype in IDH-mutant gliomas." Clinical Cancer Research (2014). (2)
  6. Fischer, Simon, et al. "Breaking limitations of complex culture media: Functional non-viral miRNA delivery into pharmaceutical production cell lines." Journal of biotechnology 168.4 (2013): 589-600.
  7. Bai, Dongmei, et al. "Regulation of the HDM2-p53 pathway by ribosomal protein L6 in response to ribosomal stress." Nucleic acids research 42.3 (2014): 1799-1811.
  8. Liu, Xing, et al. "Isocitrate dehydrogenase 2 mutation is a frequent event in osteosarcoma detected by a multi‐specific monoclonal antibody MsMab‐1." Cancer medicine 2.6 (2013): 803-814.

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